High+Low PapillomaStrip kit is based on the principle of reverse hybridization and allows for the detection and identification of 19 low risk genital HPVs in DNA samples from cervico-uterine smears or biopsies.
Human papillomavirus (HPV) is responsible for a variety of skin and mucosa disorders. To date, over 100 different HPV genotypes have been described, of which more than 40 infect the anogenital tract. Of these, approximately 1/3 is associated with cervical cancer and anal neoplasm.
• This test is based on capture with specific probes of nucleic acids of amplified fragments by the polymerase chain reaction. This capture can be done with immobilised probes either on to a membrane (OpeGen line, based on the Southern reverse blot) or with probes bounded to coloured latex particles (OligoGen line, based on the oligo-immunocromatography).
• All PCR reagents are included in the kit: Taq polymerase, primers and Master Mix.
• Several kinds of methods have been validated: salting-out, silica columns, commercial kits (automated extraction), etc.
• Strips show HPV subtypes by enzymatic color reaction visible to the naked eye.
• Minimal equipment required (thermal cycler).
• Automated protocols available for Auto-LiPA / Profiblot and AUTOBLOT instruments.
HPV subtypes Detected
19 human Papillomavirus subtypes of low risk: 6, 11, 40, 42, 43, 44, 54, 61, 62, 67, 70, 71, 72, 74, 81, 83, 84 y 91
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