High PapillomaStrip kit is based on the principle of reverse hybridization and allows for the detection and identification of 19 medium-high risk genital HPVs in DNA samples from cervico-uterine smears or biopsies.
Specific primers for E6-E7 regions for each of the 37 HPVs detected with the kit are used to perform the multiplex PCR. Since the expression of the E6-E7 genes is directly associated with carcinogenesis, detection of these targets may provide significant biological and pathological information.
• This test is based on capture with specific probes of nucleic acids of amplified fragments by the polymerase chain reaction. This capture can be done with immobilised probes either on to a membrane (OpeGen line, based on the Southern reverse blot) or with probes bounded to coloured latex particles (OligoGen line, based on the oligo-immunocromatography).
• All PCR reagents are included in the kit: Taq polymerase, primers and Master Mix.
• Several kinds of methods have been validated for DNA extraction: salting-out, silica columns, commercial kits (automated extraction), etc.
• Strips show high risk HPV subtypes by enzymatic color reaction visible to the naked eye.
• Minimal equipment required (thermal cycler).
• Automated protocols available for Auto-LiPA / Profiblot and AUTOBLOT instruments.
HPV subtypes Detected
19 human Papillomavirus subtypes of medium-high risk: 16, 18, 26, 31, 33, 35, 39, 45, 51, 52, 53, 56, 58, 59, 66, 68, 69, 73 and 82.
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